*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I haven't used any of these myself, but I've compiled a list of 
commercially 405nm excitable dyes for our users here:
http://nic.ucsf.edu/dokuwiki/doku.php?id=dyes:405nm

If you try any it would be great to get some feedback about how they 
perform.

Best,
Kurt

On 6/28/2012 3:00 AM, Graham Wright wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear all,
>
> I'm looking for recommendations for a blue dye (ex. 405nm) to be used on a
> secondary antibody. Unfortunately, the other wavelengths are already taken,
> which would normally be my suggestion.
>
> I've had a look through the archives of this list and there is confirmation
> that they are not ideal and tend to bleach easily, but are there any
> updates on this since 2008/2010?
>
> Any comments and recommendations will be appreciated about the options:
> Alexa 405, Pacific Blue, Cascade Blue...
>
> Much obliged,
> Graham
>