CONFOCALMICROSCOPY Archives

February 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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From:
Greg Erdos <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 18 Feb 1997 08:50:22 -0500
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Congo Red will work and a dye called calcofluor is also good.
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
At 03:31 PM 2/18/97 +1000, you wrote:
>Hello,
>
>Does anybody know of a fluorescent dye for demonstrating features of a
>cotton fibre?  I am looking at running a prac subject for biologists whereby
>I get them to stain textile fibres (since these are readily available) with
>fluorescent dye and look to see which structures take up the dye.  Can
>anyone tell me what is a good stain to use for cotton?  Are there any
>pitfalls I should perhaps know about in advance?  I made up a fluorescein
>dye both in water and in ethanol but had minimal luck with these.  The
>literature is quite sparse but I believe the wall of the cotton fibre to be
>made of cellulose.
>
>I no longer subscribe to this discussion group so would ask that replies be
>sent directly to my email address.  If I am overwhelmed by responses, I will
>summarise these and post them to the list later.
>
>Many thanks for any information you can supply.
>
>Felicity Lawrence
>
>Analytical Electron Microscopy Facility
>Queensland University of Technology
>GPO Box 2434, Brisbane 4000
>Australia
>
>Ph : 3864 2557
>Fax: 3864 5100
>
>
*******************************************************
G.W. Erdos, Ph.D.                  Phone: 352-392-1295
 Scientific Director,
 ICBR Electron Microscopy Core Lab
 218 Carr Hall                          Fax: 352-846-0251
 University of Florida                E-mail: [log in to unmask]
 Gainesville, FL 32611              http://www.biotech.ufl.edu/~emcl/
                     Home of the #1 Gators
*****
"Many shall run to and fro, and knowledge shall be increased"
                                                        Daniel 12:4

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