Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Without intending to clog up the list traffic, I would personally like
to express a huge THANK YOU for making this available, and I'm sure that
many, many others feel the same.
It won't keep me from ordering the new edition....;-)
James Pawley wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hi all,
>
> During the recent, prolonged discussion of the nitty-grittyof Nyquist
> sampling, I promised to make available the relevant chapter from the 3rd
> edition of the Handbook. It can now be found at
>
> http://www.springer.com/0-387-25921-X
>
> You can download a PDF by clicking on
>
> _Sample Chapter: Points, Pixels, and Gray Levels: Digitizing Image Data
> (pdf, 4.2 Mb)_
>
> on the right side. If you are looking for a lot of math, this isn't for
> you. But if you want some discussion about how the math that is usually
> rolled out is perhaps a poor fit to the noisy data we actually have to
> work with, it may be interesting.
>
> On the same page, there is also a link to a PDF of Appendix 3, on the
> operation of CCD devices, that some may find useful.
>
> Sample Chapter: More Than You Ever Really Wanted to Know About
> Charge-Coupled Devices (pdf, 912 Kb)
>
> At the risk of starting the whole story going again, I will just say
> that Chapter 4 says basically what was covered before but with some
> additional discussion of why and how the straight-forward application of
> Nyquist mathematics is complicated by the high level of Poisson Noise
> inherent in most confocal data sets and why one should always deconvolve
> confocal data before using it as the basis of anything more than the
> most cursory analysis.
>
> I promise to stop now.
>
> Cheers,
>
> Jim Pawley.
>
> --
>
> **********************************************
> Prof. James B. Pawley, Ph.
> 608-263-3147
> Room 223, Zoology Research Building,
> FAX 608-265-5315
> 1117 Johnson Ave., Madison, WI, 53706
> [log in to unmask]
> 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver
> Canada
> Info: http://www.3dcourse.ubc.ca/ Applications due by March
> 15, 2007
> "If it ain't diffraction, it must be statistics." Anon.
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