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February 2007

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Subject:
From:
Dale Callaham <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 19 Feb 2007 10:18:12 -0500
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Without intending to clog up the list traffic, I would personally like 
to express a huge THANK YOU for making this available, and I'm sure that 
many, many others feel the same.

It won't keep me from ordering the new edition....;-)


James Pawley wrote:
> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hi all,
> 
> During the recent, prolonged discussion of the nitty-grittyof Nyquist 
> sampling, I promised to make available the relevant chapter from the 3rd 
> edition of the Handbook. It can now be found at
> 
> http://www.springer.com/0-387-25921-X
> 
> You can download a PDF by clicking on
> 
> _Sample Chapter: Points, Pixels, and Gray Levels: Digitizing Image Data 
> (pdf, 4.2 Mb)_
> 
> on the right side. If you are looking for a lot of math, this isn't for 
> you. But if you want some discussion about how the math that is usually 
> rolled out is perhaps a poor fit to the noisy data we actually have to 
> work with, it may be interesting.
> 
> On the same page, there is also a link to a PDF of Appendix 3, on the 
> operation of CCD devices, that some may find useful.
> 
> Sample Chapter: More Than You Ever Really Wanted to Know About 
> Charge-Coupled Devices (pdf, 912 Kb)
> 
> At the risk of starting the whole story going  again, I will just say 
> that Chapter 4 says basically what was covered before but with some 
> additional discussion of why and how the straight-forward application of 
> Nyquist mathematics is complicated by the high level of Poisson Noise 
> inherent in most confocal data sets and why one should always deconvolve 
> confocal data before using it as the basis of anything more than the 
> most cursory analysis.
> 
> I promise to stop now.
> 
> Cheers,
> 
> Jim Pawley.
> 
> -- 
> 
>               **********************************************
> Prof. James B. Pawley,                                          Ph.  
> 608-263-3147 
> Room 223, Zoology Research Building,                                  
> FAX  608-265-5315
> 1117 Johnson Ave., Madison, WI, 53706                                
> [log in to unmask]
> 3D Microscopy of Living Cells Course, June 17-28, 2007, UBC, Vancouver 
> Canada
> Info: http://www.3dcourse.ubc.ca/            Applications due by March 
> 15, 2007
>        "If it ain't diffraction, it must be statistics." Anon.

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