it's possible to control the microscope remotely, keeping both the
sample and user in optimal environments
/Johan
George McNamara wrote:
> Hi Shawn,
>
> Windows for a fluorescence microscope facility are a waste of good
> office or general lab space.
>
> Temperature: I keep our Zeiss LSM510/UV confocal microscope room as
> cool as possible, ~20 C. I occasionally have users who mention the
> temperature. My response is that (1) I have measured the output of the
> lasers (and/or AOTF intensity control or other components) and they
> are only stable when the room is cool, and (2) I would be happy to add
> UM sweater rental to my price list if they'll pay the rent.
>
> If the PI likes the data you generate, they can motivate the depressed
> user or find a new employee.
>
>
> George
> p.s. none of the authors of the three Nature papers who published
> LSM510 data in the last two years complained about our windowless core
> being depressing or cold.
>
>
> At 02:59 PM 10/26/2009, you wrote:
>> These comments bring up a question I've long had regarding the
>> intersection between the practice of microscopy and the business of
>> running an imaging facility: Windows -- not the OS, but the objects
>> themselves.
>>
>> It seems to me that one way to encourage return visits to a facility
>> is by providing an environment that users find inviting, or at least
>> not frightening. One easy way to do this is to have analysis areas
>> or main lab spaces that are windowed. However, every room with
>> windows is one that is necessarily restricted in terms of function (as
>> Craig and Ammasi mention). While I understand that scientific
>> concerns are paramount and questions of ambience should not matter,
>> I've had more than one experience where users have told me that they
>> would not return to a facility because it was 'too depressing'. Some
>> may argue that what is depressing is their commitment to their
>> projects, but we have little choice concerning motivation.
>>
>> I don't know that there's a right or wrong answer to windows in a
>> facility, but could some of you with more experience comment on the
>> net benefit/cost? It would help me (and hopefully others) when
>> thinking about such designs in future.
>>
>>
>> Thanks,
>>
>> Shawn
>>
>>
>> Shawn Galdeen, Ph.D.
>> Research Support Specialist
>> Bio-Imaging Resource Center
>> Rockefeller University
>> 1230 York Ave.
>> New York, New York 10065
>> 212.327.7487
>>
>>
>> -------------------------------------------------------------------------------------
>>
>> On Oct 26, 2009, at 2:19 PM, Craig Brideau wrote:
>>
>> If you have control over the construction/renovation of the room;
>> don't put windows in it!
>>
>> Craig
>>
>> ------------------------------------------------------------------------------------
>>
>> On Mon, Oct 26, 2009 at 12:15 PM, Periasamy, Ammasi (ap3t)
>> <[log in to unmask]> wrote:
>>> Hello
>>> I always prefer the basement for microscopy facility. In the
>>> basement or ground floor you will not have floor vibration issue and
>>> also it provides light proof if you would like to do lifetime
>>> imaging. Otherwise, you have to hang curtains, etc., to cover the
>>> light leak. First floor is ok, all other floors are not ok because
>>> of vibration of the floor if someone hammer the wall the confocal
>>> scanner will go crazy.
>>> Right now our center is in the ground floor (13 years) and we are
>>> planning to move to the new building ( more space!!)in the basement
>>> in another two years.
>>> You may miss the daylight but it's good for the microscope system if
>>> it is protected well regarding all the water leaks, etc.
>>> Hope this helps.
>>>
>>> Ammasi Periasamy, Ph.D.
>>> Director, Keck Center for Cellular Imaging (KCCI)
>>> Professor of Biology and Biomedical Engineering
>>> Biology, Gilmer Hall (064), McCormick Rd
>>> University of Virginia
>>> Charlottesville, VA 22904
>>> Voice: 434-243-7602 (Office); 982-4869 (lab)
>>> Fax:434-982-5210; Email:[log in to unmask]
>>> http://www.kcci.virginia.edu
>>> ************************
>>> Workshop on FRET Microscopy, March 9-13, 2010
>>> http://www.kcci.virginia.edu/workshop/workshop2010/index.php
>>> *************************
>
>
>
>
>
>
>
> George McNamara, Ph.D.
> Image Core Manager
> Analytical Imaging Core Facility
> University of Miami, Miller School of Medicine
> Miami, FL 33136
> [log in to unmask]
> [log in to unmask]
> 305-243-8436 office
> http://www.sylvester.org/AICF (Analytical Imaging Core Facility)
> http://www.sylvester.org/AICF/pubspectra.zip (the entire 2000+ spectra
> .xlsx file is in the zip file)
> http://home.earthlink.net/~geomcnamara
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