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Tim,

The latest versions of ImageJ have a non-destructive overlay feature that allows one to add text and drawn objects onto an image and turn these on and off on the image at will. See documentation here:

http://rsbweb.nih.gov/ij/docs/guide/146-11.html#toc-Section-11

Cheers,

John Oreopoulos
Staff Scientist
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2013-01-08, at 10:09 AM, Tim Feinstein wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> ImageJ can 'burn' shapes, but text is awkward and I do not know an easy way to change or remove annotations after the fact like you can using 'layers' in Photoshop or GIMP.  Like most proprietary microscope software, the Nikon Elements that we use has an annotation package that works well for basic stuff and it all goes on a removable 'layer'.  Like others I'd recommend starting with whatever comes with the scope.  
> 
> all the best, 
> 
> 
> TF
> 
> Timothy Feinstein, PhD
> Visiting Research Associate 
> Laboratory for GPCR Biology
> Dept. of Pharmacology & Chemical Biology
> University of Pittsburgh, School of Medicine 
> BST W1301, 200 Lothrop St.
> Pittsburgh, PA  15261
> 
> On Jan 8, 2013, at 9:29 AM, Cammer, Michael wrote:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Zeiss Zen software has annotation tools built in as noted below.  
>> 
>> I also keep ImageJ on all microscope platforms.  Haven't had any conflicts.
>> 
>> _________________________________________
>> Michael Cammer, Assistant Research Scientist
>> Skirball Institute of Biomolecular Medicine
>> Lab: (212) 263-3208  Cell: (914) 309-3270