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Hi,

I have been looking for the plug-ins you describe and cannot seem to find any
by that name.  Where are they?

Thanks for the info.

Deb

Karl Garsha wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> One thing to keep in mind is that multicolor images from the confocal
> microscope are (usually) 8-bit grayscale images colored with lookup tables
> by the confocal software.  I like to use imageJ to make the initial .avi
> movie files (with the .avi writer plugin) and then you can compress the
> movie and change the format using Quick time Pro.
>          With the new leica format, the individual channels in a stack are
> already separate.  With the older leica multi-tiff format you'll need to
> separate the channels into separate multi-tiffs, and then either convert
> the multitiffs into individual frames for import as an image series or open
> the multi-tiff stacks using the Leica SP reader plugin.  (ImageJ has two
> plugins which are useful for the older Leica file format: Leica SP stack
> splitter and Leica SP reader-first split the stack, then read the
> individual stacks).  Next you want to use the Plugins->color->RGB stack
> merge plugin to convert your individual 8-bit stacks into a 24-bit color
> (RGB) image.  To do this you just select the plugin from the menu and it
> will ask you which stack (channel) should be red, which blue, and which
> green.  If you only have two channels then you select 'none' for one of the
> color channels.  The result of this activity will be a 24bit RGB image
> stack which is what you need (in many cases) for making a color movie of
> your confocal data.  Finally use the Plugins->input/output-> .avi writer
> plugin.  You will end up with an .avi (windows animation file) which can be
> directly imported into your power-point presentation, however the file will
> likely be quite large (depending on the resolution of your
> data).  Quicktime pro can be used to fine tune your movie (speed/size) you
> might want to convert the .avi file to a Quicktime movie (.mov), which uses
> an efficient compression algorithm.  One major caveat: I've never had much
> luck importing a quicktime movie into a powerpoint presentation-I'm sure
> there is a way to do this, and it may just be my version of power-point or
> the compression settings I used, but I have always ended up being forced to
> use an uncompressed .avi file (30-50megabytes or more).  You'll need a
> reasonably decent laptop to pull this off in a presentation.  Also, make
> sure the movie file is in the same directory as the powerpoint presentation
> for the presentation-otherwise the presentation will not be able to play
> the movie.
>          Hope this helps,
>          Karl G.
>
> At 10:34 AM 3/19/2002 -0700, Deb Berglund wrote:
> >Search the CONFOCAL archive at
> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >Hi,
> >
> >I have some Leica stacks that I would like to generate into movies that I
> >can use
> >for a powerpoint presentation.  Does anyone know how to do this?  All I
> >want is to
> >flip through the slices, be able to control the speed, and be able to use
> >software
> >that can be carried to a PC that does our powerpoint control of a
> >projector.  The
> >stacks are from Leica's older software, but I can split the slices into
> >individual
> >tif images with tifsplt.
> >
> >Thanks in advance.
> >
> >Deb Berglund
> >Montana State University
>
> _______________________________________________
> Karl Garsha
> Light Microscopy Specialist
> Imaging Technology Group
> Beckman Institute for Advanced Science and Technology
> University of Illiniois at Urbana-Champaign
> 405 North Mathews Avenue
> Urbana, IL 61801
> Room B650J
> Tel: (217) 244-6292
> Fax: (217) 244-6219
> www.itg.uiuc.edu