Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Chris; You can "roll your own" anti fade with the recipe at the following URL: http://www.fhcrc.org/shared_resources/imaging/DABCO.html Regards, Tom Tom Donnelly Applied Precision, LLC Biotechnology Group 1040 12th Ave. N.W. (425)657-1294 Issaquah, WA 98027-8929 (425)557-1055 fax [log in to unmask] http://www.api.com/products/bio/deltavision.html -----Original Message----- From: Chris Jones [mailto:[log in to unmask]] Sent: Monday, October 28, 2002 5:45 AM To: [log in to unmask] Subject: Anti-fade mounting media Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal What experiences have you had using the various mounting media containing anti-fade reagents? Our usual fluorochromes are FITC, Cy3, and Lucifer Yellow and we have been using VectaShield to mount, but lately have been having major fading problems. Thank you.