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Hi Shalin, 

Using the same hardware we have also found the escape button to be quite
risky between users.  Of equal concern is preserving saved XYZ positions.
We have had to replace expensive bits (but not yet an objective, knock on
woodŠ) when the stage automatically tries to return to a previous user¹s
XYZ position that was set with a very different stage insert installed.
As a result we ask all users to manually lower the objectives and delete
their saved XYZ positions when finished.

All the best, 


TF

Timothy Feinstein, Ph.D. | Confocal Manager
333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
Phone: 616-234-5819 | Email: [log in to unmask]







On 6/17/14, 11:20 AM, "Sylvie Le Guyader" <[log in to unmask]> wrote:

>Hi Shalin
>
>I assume that this happens because one user escapes the objective at the
>end of the imaging session and leaves the system on for the next user who
>then presses the reload button.
>Indeed I do not understand why Nikon has chosen to lock the focus wheel
>when the objective has been escaped so that the only way to get in focus
>again is to reload.
>
>In the meantime I would suggest you do as us: tell users to
>systematically shut down the software and hardware (leaving only the gas
>lasers on) between 2 users. This way when the next user turns the system
>on (it only takes a minute), the objective will be automatically escaped
>and the focus wheel will be unlocked.
>
>Hope that helps :)
>
>Med vänlig hälsning / Best regards
> 
>Sylvie
> 
>@@@@@@@@@@@@@@@@@@@@@@@@
>Sylvie Le Guyader
>Live Cell Imaging Unit Manager
>Dept of Biosciences and Nutrition
>Karolinska Institutet
>Hälsovägen 7
>Novum, G lift, floor 6
>14157 Huddinge
>Sweden
>office: +46 (0) 8 5248 1107
>LCI room 1: +46 (0) 8 5248 1172
>LCI room 2: +46 (0) 8 5248 3542
>mobile: +46 (0) 73 733 5008
>
>
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:[log in to unmask]] On Behalf Of Shalin Mehta
>> Sent: 17 June 2014 16:47
>> To: [log in to unmask]
>> Subject: Limiting the z travel range on Nikon Ti
>> 
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>> 
>> Hello listers,
>> Our lab has damaged two objectives so far by running them into stage on
>>our
>> inverted Nikon Ti by using the 'Escape' and 'Return' for Z.
>> 
>> Our old Nikon (finite tube length) has a mechanical stop on how close
>>the objective
>> can come to the stage and all old objectives are in great shape.
>> 
>> Can we program the limits on Z travel on Nikon Ti? Asking our local
>>Nikon folks
>> suggests that the answer is No and we should avoid using escape/return.
>> 
>> If this feature is not in the firmware, this is a request to Nikon
>>Japan for such a
>> feature.
>> 
>> Thanks
>> Shalin