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September 2012

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Subject:
Re: Background fluorescence problem
From:
Matt Kofron <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 14 Sep 2012 09:02:11 -0500
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Have you tried a narrow YFP filter? A 500/20 excitation filter should still
excite GFP but shift away from the flavin peak. You will see less GFP, but
it might improve S/N.

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