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Most deconvolution microscopy is concerned about fluorescent samples.
However it is possible to processes laser scanning transmission images in a similar
fashion. Those images are usually obtained by measuring the intensity of the
transmitted laser beam in a non-descanning fashion. Best contrast is obtained by
stopping down the condenser because fluctuations in the refractive index of the
sample make it appear similar to phase contrast microscopy.

Such transmission data is not optically sectioned like confocal or 2P images. There
are several publications about using deconvolution approaches for transmission
electron microscopy.

I would like to ask if anyone is aware of publications or work conducted on
"deconvolving" optical transmission or phase contrast data stacks in order to
improve Z resolution.

Urs Utzinger
University of Arizona