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Hi Jeff,

in our facility, we are pretty happy with the stage-top incubator system called 
"Chamlide TC" by Live Cell Instruments, distributed by Quorum Technologies. 
[No commercial interest, just a happy customer.] The chamber can 
accommodate a number of different sample holder types (small glass-bottom 
dishes, round cover glasses in a magnetic reusable holder, rectangular multi-
well dishes of slide size), and controls temperature, CO2 and humidity.
Since the imaging is through a regular cover glass (or glass-bottom dish), the 
objectives on an inverted scope should work just like with a slide plus cover 
glass. I'm not sure what you mean using the long working distance objective in 
the "forward detection". Condensers can get pretty close to the top of the 
incubator chamber, if that is your concern for the transmitted light setup. But 
on our spinning disk systems (Quorum WaveFX on Leica DMI6000B), this 
hasn't been a problem.

Elke

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Elke Küster-Schöck
CIAN (Cell Imaging and Analysis Network)
Proteomics & Genomics Coordinator, Microscopy Associate
McGill University, Stewart N5/25
514-398-5414
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