Dear Listmembers,

I recently tried to image (H33342,nuclei stained) brain slices for a
colleague.  The slices were 200micron thick but we could only get 'good'
imaging down to about 40 micron.  I dont have much experience of this type
of tissue and I wonder if any of you have managed get anywhere near
100micron penetration on either fixed or living brain slices.

Has anyone estimated the refractive index of brain tissue?

Thanks

Craig.

______________________________________________________________________________
Craig J. Daly
Research Associate                     tel:       0141 330 6606
Autonomic Physiology Unit (APU)        fax:       0141 337 1651
West Medical Building                  email:     [log in to unmask]
University of Glasgow
Glasgow G12 8QQ.

Web Sites                             http://www.cardiovascular.org
                                      http://www.adrenoceptor.com
_____________________________________________________________________________