hello Bruno SAUBAMEA
what i could see from my stainings, it seems that the background is so  
high that the specific signal canot be seen. to get rid of the high  
background i tried washing with high salt PBS but it seems to have  
done no improvement to my samples.
  thank you
  Shuchi Gupta
Rudolf Virchow Centre for experimental medicine.
Uni Wuerzburg
Germany




Quoting Bruno Saubaméa <[log in to unmask]>:

> Dear Shuchu,
> what do you think is the problem :the bg is low but you don't have a  
> specific signal or the bg is so high that the specific signal can't  
> be seen?
> bruno
>
> Bruno SAUBAMEA
>
> EA 3621 & Service Commun d'Imagerie Cellulaire et Moléculaire
>
> Faculté des Sciences Pharmaceutiques et Biologiques
> Université Paris Descartes
> 4, avenue de l'Observatoire
> 75006 PARIS
>
> tel : 01.53.73.97.13
> fax : 01.53.73.99.09
>   ----- Original Message -----
>   From: Shuchi Gupta
>   To: [log in to unmask]
>   Sent: Monday, March 09, 2009 5:23 PM
>   Subject: secondary antibody from immunostainigs
>
>
>   hello,
>     i want to know if somebody knows any good secondary antibodies(
>   alexa, atto conjugated) against rabbit which i can use for the
>   immunostainings. i have tried  antibodies from cell signaling, abnova
>   and dako.but none of them give me the specific signal .( this i get to
>   know from my controls which contain only my secondary antibody) .  i
>   am using these antibodies in mouse platelets.i block them with BSA 2%
>   after fixing and permeabilization.
>   hope to get the suggestions soon.
>   thank you
>   shuchi
>