Hi Robert,
Yes, most of coverglass is quite dirty (most of standard #1.5 coverslips
from many suppliers, even the German one from the Hecht_Assistent; also
very dirty BK-7 coverglass, Nuncbrand LabTek I or II, Grace Biolabs 16-well
chambered coverglass). The MatTek glass bottom dishes are cleaner but in
many instances the coverglass is "angled", e.g. mounted not strictly
horizontal giving you out-of-focus corners when you image anything that
mounted on the surface.
I attached one rare paper from China that is surprisingly very remarkable,
in technical terms. ... as NIH would never fund such a project (but USDOE
might have contributed to the study).
It is a shame that postdocs (and/or skilled technicians) have to kill time
cleaning coverglass... Maybe NIH can provide funds for a "Coverglass
Cleaning Biotech Startup Company"
Good luck,
Vitaly
NCI-Frederick
301-846-6575
PS As my original posting was rejected 'cos I attached pdf file, I will send
it to upon request
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of [log in to unmask]
Sent: Wednesday, June 24, 2009 7:44 PM
To: [log in to unmask]
Subject: Clean slides and cover slips
I was using some good quality pre-cleaned microscope slides and noticed
that they are not clean but very "dirty". Good cover slips ( 1.5 )
also have some dirt on them.
Does anyone have a protocol for cleaning precleaned slides and
coverslips and a source to perhaps buy good slides/coverlsips. .
It is very hard to look at small particles in a field with dirt and
streaks on the so called pre-cleaned slides
thanks
bob
Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory.
Reproductive Toxicology Division
Telephone: 919-541-1585 Fax: 919-541-4017
e-mail: [log in to unmask]
Mail address: USEPA,ORD,NHEERL,RTD
Developmental Biology Branch ( MD 67)
Research Triangle Park, North Carolina, 27711
Shipping address:
2525 E.NC Highway 54
Durham, NC, 27713
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