CONFOCALMICROSCOPY Archives

October 2009

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Subject:
From:
"MODEL, MICHAEL" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 7 Oct 2009 16:30:11 -0400
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This is one thing we haven't checked! Very interesting, thank you!

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Rosemary White
Sent: Wednesday, October 07, 2009 4:18 PM
To: [log in to unmask]
Subject: Re: exorcising spirits from Fluoview

We had a very similar problem, but it turned out to be the connection
between the fibre-optic cable and the laser - it had come slightly loose.
Since you've checked the cables, I guess it has to be something else.

good luck,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334


On 7/10/09 10:27 PM, "MODEL, MICHAEL" <[log in to unmask]> wrote:

> I apologize if this is a second message to the list, I think the first one
> didn't go through
>
> We are having a bizzare scanning problem. Straight vertical lines in an object
> become slightly zigzagged with a period of up to 6-7 scan lines, and there
> also may be some oscillation in the intensity. The period and the magnitude of
> this periodic noise depends on the scan speed and the scan size. So far we
> (with the help of an Olympus engineer) have established that:
>
> 1. It doesn't seem to be the scanner controller or the galvo mechanism
> 2. It does not seem to be the electric power in the building
> 3. It is not a mechanical vibration
> 4. It is not a computer
> 5. It is not the cables
> 6. It is not a 60 Hz noise
>
> Sometimes connecting the scanner controller to the outlet through a long
> extension cord seemed to help which may suggest a problem with grounding, but
> as soon as we concluded that, the trick stopped working. The trouble could not
> be reproduced at the Olympus testing lab.
>
> Has anyone experienced anything similar and successfully resolved the problem?
>
> Many thanks in advance!
>
> Mike Model

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