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REMINDER as early booking prices are only available for a few more days.

CONFOCAL MICROSCOPY
4-Day Intensive Hands-On Course

All enquiries please contact Peter O'Toole ([log in to unmask]).

Website: http://www.york.ac.uk/biology/tf/

Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing

16th-19th September 2003
Technology Facility, Dept. Biology
University of York, UK

Instructors will include

Peter O'Toole, University of York
Les Borland, Carl Zeiss
Andrew Hubbard, Perkin Elmer
and special guest lecture by Daniel Zicha, Cancer Research UK.

Tutorials and practical sessions will cover both basic and advanced
confocal microscopy. The course will enable participants to realise the
potential behind many developing confocal techniques and realise the
simplicity of applying the techniques to a broad range  of sample types
and fluorescent labels. Both novice and more experienced users welcome.

Preliminary Programme
DAY             AIMS
Day 1:  Basics, Familiarisation, x,y,z and multicolour imaging.
Day 2:  Live-cell imaging with spinning disk confocal microscopy, FRAP
on laser scanning confocal.
Day 3:  Advanced Techniques covering spectral unmixing and FRET with
fluorescent proteins.
Day 4:  Further advances. More samples to explore confocal limits, and
time for participants own samples.

Thursday evening will include a special guest speaker (Daniel Zicha,
Cancer Research UK) and course meal.

The course will utilise many different sample types ranging from
cultured monolayers to plant cells. A diverse range of fluorescent
labels will also be used, which will include various fluorescent
proteins (CFP, GFP and YFP) and classic antibody labels. Over the four
days, three confocal microscopes (2x Zeiss LSM 510 meta scan heads on
both an invert and upright microscope, PerkinElmer UltraVIEW) will be
utilised.

Accommodation (en-suite), breakfast and lunches will be provided for the
length of the course.  Evening meals will be provided on both Tuesday
and Thursday evenings.

For further information, please visit the course website at:
http://www.york.ac.uk/biology/tf/
  or contact Margaret Newton 01904 328821, [log in to unmask]


--
Dr Peter O'Toole
Imaging and Cytometry Laboratory Manager
Technology Facility
Department of Biology (Area 15)
University of York
PO Box 373
YORK
YO10 5YW

Tel : +44 (0)1904 328722
Fax : +44 (0)1904 328804
email : [log in to unmask]
www.york.ac.uk/biology/tf