> Does anyone know the excitation and emission peaks
> of Evans Blue?  Better yet, does anyone have a graph
> of its excitation and emission spectrum?
> Alternatively, if someone is currently using a filter set (of
> any manufacturer's brand) to visualize Evans Blue in
> fluorescence, could you provide me with that part number?

Jenny,
extracts from the confocal archives....

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
>>> Posting number 6023, dated 4 Oct 1996 13:37:44
Rhodamine-like works. Whether it is optimal or not I don't know.

>>> Posting number 6024, dated 7 Oct 1996 07:41:43
"Reported spectra: Abs 600-620 nm, Em about 680nm" ... "Em about 680
nm; broad excitation peak, 450-550 nm; Em 670 nm; Abs 511nm".  From
Fluorescence Microscopy vol 2 by F.W.D. Rost. IT looks like it would
work with a KrAr laser and a 568 ex/ 680/32 em filter combination.
                                                                George
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

The Nikon fluorescence brochure also states excitation as green and
recommedns their emisson filter sets G-2A  (ex 510-560, Dichroic 580,
em LP590) and G-1B (ex 546/10, dichroic and em as sabove).

Hope this helps,

Ian.

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Dr Ian S. Harper
Confocal Microscopy Facility
Department of Biological Sciences
Monash University, Clayton, Vic 3168, Australia
Email: [log in to unmask]
Tel: +61 3 9905 5635;  Fax: +61 3 9905 5613
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