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Dear Monique,

I've been using a perfluorocarbon mounting medium for in vivo imaging of leaf tissue for a few years now (Littlejohn, G. R., Gouveia, J. D., Edner, C., Smirnoff, N. and Love, J. (2010), Perfluorodecalin enhances in vivo confocal microscopy resolution of Arabidopsis thaliana mesophyll. New Phytologist, 186: 1018–1025. doi: 10.1111/j.1469-8137.2010.03244.x). In addition we often use Carolina observation gel for mounting leaf samples. 

We have an online protocol here: http://www.jove.com/video/3394/a-simple-method-for-imaging-arabidopsis-leaves-using-perfluorodecalin-as-an-infiltrative-imaging-medium
Hope it helps!

All the best,
George

******************************
Dr. George Littlejohn
School of Biosciences,
University of Exeter,
Mezzanine Laboratory,
Geoffrey Pope Building,
Stocker Road,
EX4 4QD, UK
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Tel:  +44(0)1392 269170 (Lab.)
        +44(0)1392 269297 (Office)
Fax:  +44(0)1392 263434
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http://www.illuminatedcell.com/improved-imaging.html
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From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Vasseur Monique [[log in to unmask]]
Sent: Tuesday, January 24, 2012 9:31 PM
To: [log in to unmask]
Subject: Plant preparation for confocal microscopy

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Dear all,

Do some of you could suggest how to prepare live sample of plant leaf for
confocal microscopy.  We don't have much idea how to "sandwich" a leaf
between slide and coverslip so that it is flat et not too thick for microscopy
(and without destroying it)? What should we use as mounting media? What
should we be aware of?  Our plant will have YFP in chloroplastides.

Thanks a lot in advance to all of you.

Monique