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I think that Fit_Polynomial in ImageJ might do what you want.
https://imagejdocu.tudor.lu/plugin/filter/fit_polynomial/start
There is also a package called Gwyddion
http://gwyddion.net/
That has a rich collection of leveling methods, including the ability to
create a polynomial background interpolated from the masked areas only.
It is geared towards scanning probe microscopy, but it may do what you want.
best regards
--aryeh
On 24/11/2021 17:22, Model, Michael wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List,
>
> Does anyone know how to subtract an uneven background from an image with large objects and small background area? Fully automated methods (rolling ball) would not work on such images. Ideally, I would like to manually pick a number of points that I think represent the background in various parts of the image and then run a 2D interpolation. Then the interpolated image can be subtracted from the original image. I think this problem has been resolved in Photoshop but I don't know if it works on >8 bit images.
> Thank you!
>
> Mike
--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel
Ph: 972-3-5317638
FAX: 972-3-7384051
