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Hi, Ian,

Thank you very much for your kind posting.  

It looks like that we will have to avoid this dye in the future.  Too much 
trouble.  We do use a lot of Alexa 555 and Alexa 594 in the lab.

Have a great day.

Jasmine


Quoting "Clements, Ian" <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Over the years I've tried and developed a range of different antifade
> cocktails and unfortunately I can't say that any of them worked
> particularly well for R-PE.  None of the commercial formulations I've
> tried work better than the home mades, which is to say not very well
> overall.  
> 
> You can image R-PE if you can do all you initial focusing on a different
> channel, but you'll probably get one shot at the R-PE unless its very
> abundant so repeated scanning of this channel is usually not possible.
> 
> If you want something in this color range try Alexa Fluor 555 instead.
> 
> Commercial interest with Molecular Probes/Invitrogen related products
> acknowledged.
> 
> Ian Clements
> Invitrogen Corp
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Xinyu Zhao
> Sent: Thursday, May 25, 2006 11:34 AM
> To: [log in to unmask]
> Subject: Mounting medium for R-PE
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Dear listers,
> 
> I was wondering if anybody can suggest a mounting medium which can
> preserve 
> R-PE nicely.   We have serveral users using this fluorochome and mount
> the 
> slides in Fluoromount-G and Vectorshield respectively.  In both cases,
> the 
> signal faded very quickly, especially with Fluoromount-G.  Really
> frustrating.
> 
> Thank you very much.
> 
> Jasmine
> 
> Biomedical Imaging Core 
> School of Medicine
> University of Pennsylvania
> Philadelphia, PA 19104
> 


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