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April 2011

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Subject:
Live cell imaging with multiphoton confocal
From:
David Knecht charter <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 22 Apr 2011 13:29:03 -0400
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I have limited experience with multi-photon confocal microscopes.  I tried imaging live cells with one a while back and was unimpressed with the results as compared to a standard laser scanning instrument.  If you had a new single photon instrument and a new multi-photon instrument sitting side by side in your facility (and I presume some of you do), which would you choose for imaging  fixed cells or live cells in culture (not trying to go deep into tissue)?  Thanks- Dave

Dr. David Knecht    
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

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