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October 1995

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Subject:
Re: dual dye calcium
From:
Ray Hicks <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 27 Oct 1995 11:08:38 +0000
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Hi,
I can't comment on the dye you're proposing, but there is a published
method using 488nm excitation of SNARF read at its isosbestic point of
around 620nm (this dye is in itself a pH-dependent ratio emitter, but the
isosbestic point doesn't change in intensity), the reference is
"Improved method for Measuring Intracellular Ca++ with Fluo-3"G.T.
Rijkers,et al. Cytometry 11, 923 (1990).
The readout was flow cytometric, but should adapt to your setup.
 
Ray

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