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Regarding the question on the first paper, I think you'll need to go back to Marvin Minsky's PhD thesis in 1955. I couldn't find the text, but this is a nice read anyway

http://web.media.mit.edu/~minsky/papers/ConfocalMemoir.html

(slightly off topic: here's the patent he filed in 1957)

https://www.google.com/patents/US3013467?dq=minsky&hl=en&sa=X&ei=4N7nUuzOB4iIyAOV4IDQAQ&ved=0CDoQ6AEwAQ

Best
Diego


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Diego Morone
Research Center
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of MODEL, MICHAEL
Sent: martedì 28 gennaio 2014 17:56
To: [log in to unmask]
Subject: Re: At what confocal pinhole size does a confocal image cease to be confocal?

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Hi John.
There is a chapter about pinhole size in the 2nd edition of the Handbook (Sandison et al, pp. 39-53), and you may also check out Wilson's papers (Confocal Microscopy, Academic Press, 1990, ed. T. Wilson; chapter 1).
Best wishes

Mike Model

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of John Oreopoulos
Sent: Tuesday, January 28, 2014 11:28 AM
To: [log in to unmask]
Subject: At what confocal pinhole size does a confocal image cease to be confocal?

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I'm wondering if the listserver can help me recall something I've heard several times before and point me to a reference that proves (and demonstrates) it. Several people I think have said before (I'm looking in the direction of Jim Pawley and Guy Cox perhaps) that beyond a certain confocal pinhole size (in terms of Airy units), the image that is produced by the confocal microscope is equivalent to that which would be obtained with a regular widefield epifluorescence microscope without confocal optics.

1. Can someone tell me at what confocal pinhole size this happens?
2. Can you also tell me where this was shown in the confocal literature for the first time (or a equivalently in a review article on same the topic)?
3. Does this principle also apply to spinning disk confocal microscopes, why or why not?

Thank you greatly for your wisdom.


John Oreopoulos
Staff Scientist
Spectral Applied Research Inc.
A Division of Andor Technology
Richmond Hill, Ontario
Canada
www.spectral.ca