>Mike,
>        By the way, was this on the MRC 1000 or the MRC 600?
>                                Harvey
>
>>On the BioRad unless you have one that delivers the laser via a fiber you
>>will need a 1/4 wave plate to rotate the laser beam for good DIC.  We had
>>the same experience and obtaining a 1/4 wave plate from BioRad solved the
>>problem.
>>
 
 Harvey,
 
This was on a MRC 1024.  We use a Nikon inverted scope so the 1/4 Wave plate
is underneath the objective turrent. It is on a slide so the plate can be
removed from the optical path. I usually leave it in its apparrent
attentuation of the fluorescent signal is minimal.
 
Mike Esterman
Scientific Imaging Center
Lilly Research Labs
Indianapolis IN 46285
[log in to unmask]