Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

It might help to put the tissue or cells under a bright light during 12 to
24h before staining.  This way you'll bleach the autofluorescence (but it
might return after a while).  I tried it on muscle tissue, I also tried it
with a bright flash: I flashed it about 10-15x with high power and the
autofluorescence was reduced, but putting it under a bright lightbulb for
24h gave better results.
Good luck,

Sven Terclavers


At 10:23 3/10/2002 +0100, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Does anybody know a technique to reduce or remove
>autofluorescense retinal pigment epithelium in cryostate sections
>
>Thanks Bob
>Bob Nunes Cardozo
>Netherlands Ophthalmic Research Institute
>Retinal Signal Processing
>Meibergdreef 47
>1105 BA Amsterdam
>The Netherlands
>tel: (31)20 5665487
>fax: (31)20 5666121
>mail: [log in to unmask]