Doug

Two things come to mind:

1) Did you use plasticine "feet" on the coverslip? When using square 
coverslips you can streak each corner over a piece of plasticine leaving 
a bit of it attached to the corner of the glass. This gives the 
coverslip a support to prevent squeezing of the liquid and subsequent 
moving of the trapped medium underneath. It is quite useful when 
operating with immersion oil.

2) Some cell types' adherence capacity is temperature dependent. HEK293 
for instance detach as soon they come out of the 37 degree incubator.

Cheers

Franco

[log in to unmask] wrote:
> Last week I had some users bring in suspension cells for me to image 
> with our confocal.  Darn things were moving so much that it was 
> difficult to get two colors to line up using sequential scanning.  
> Apparently these are non-adherent cells, so growing them on a coverslip 
> is not an option.  Any suggestions?  What they gave me was a drop of 
> cells in culture media, we put it between a slide and  coverslip.
> 
>  
> 
> Doug
> 
>  
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> Douglas W. Cromey, M.S. - Assistant Scientific Investigator
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