CONFOCALMICROSCOPY Archives

November 2009

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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From:
Mark Cannell <[log in to unmask]>
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Date:
Thu, 12 Nov 2009 07:29:38 +1300
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Hi

To raise another concern, what about  UV damage to the micro lenses?

Mark.

Richard Berman wrote:
> Dear Renato:
>
> Your timing is pretty good. Over the next few months we intend to 
> explore the potential of using the Yokogawa CSU at 375nm. For our 
> testing we will use the CSU-10 although the results should apply to 
> the X1 as well. Although the wavelength is not as low as 355nm, it is 
> lower than any previous work of which we are aware. You are correct to 
> question the transmission of the disks but there are other concerns 
> too, mostly on the excitation path. The transmission of the standard 
> fiber will be very poor at 375nm and could have a shorter lifetime 
> when used with such low wavelengths. We will investigate other fiber 
> materials. We are concerned that the chromatic shift in the 
> collimating lenses and the lenslets themselves could introduce 
> problems.  In addition, there are the usual concerns about the 
> transmission efficiency of the scope and objectives. A special CSU 
> dichroic will be required to make this possible too.
>
> From our side we would like to hear from you or other CSU users about 
> the potential benefits of 375nm excitation light. For your application 
> is it necessary to get down to 355nm or is 375nm useable? Operating at 
> 355nm may be possible although it is not on our current test plans.
>
> Regards, Richard
>

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