Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Anita,

You should set your z step according to the Nyquist sampling rate.

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Wijnholds, Anita
Sent: Wednesday, December 17, 2003 2:47 AM
To: [log in to unmask]
Subject: resolution vs. analysis time

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear all,

We are measuring the development of biofilms. The z-step is calculated
according to the resolution of the lens. In first instance, we get only
a
few slices as the film is not so thick. But the film grows to more than
50
micron and so we get a lot of slices with that resolution.The analysis
of
the stacks is going to take too much time.
Can we reduce the amount of slices? What are criteria to do so?
I hope you can give me some good thougts and ideas about this.

Kind regards,

Anita Wijnholds
NIOO-CEME