CONFOCALMICROSCOPY Archives

August 1992

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Paul Goodwin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 13 Aug 1992 07:23:57 -0700
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On Thu, 13 Aug 1992, Charles F. Thomas wrote:
 
> Paul,
>
> I believe what Jim was saying was that, because most 3D reconstruction
 computers
> assume a slice is as thick in the z direction as a pixel in the x-y direction,
> you should multiply it by 6 times to make the z direction thickness closer to
> what he has measured as real.
>
> I hope he didn't say that his z resolution was 180nm, I believe that he said
 his
> Z increment (step size) was 180 nm.  Our measured z resolution with a point
> source fluorescent specimen on our MRC-600 is about 570 nm.
 
 
Thanks for the clarification. I still think we need a new word for the
rendering resolution vs actual resolution.
 
Paul

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