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September 2011

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Subject:
phenol red vs. hardware autofocus eg Nikon Perfect Focus (PFS) - near IR 870 nm LED.
From:
Daniel James White <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 7 Sep 2011 09:35:36 +0200
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Dear All prefect focusers,

We believe we see a correlation between use of phenol red
and Nikon perfect focus (870 nm), hardware autofocus slowly drifting off (over many hours)

Does anyone else have any experience with phenol red causing problems
with hardware autofocus?

cheers

Dan


Dr. Daniel James White BSc. (Hons.) PhD

Leader - Image Processing Facility,
Senior Microscopist,
Light Microscopy Facility.

Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

+49 (0)15114966933 (German Mobile)
+49 (0)351 210 2627 (Work phone at MPI-CBG)
+49 (0)351 210 1078 (Fax MPI-CBG LMF)
chalkie666 					Skype
http://www.bioimagexd.net 	BioImageXD
http://fiji.sc					Fiji -  is just ImageJ (Batteries Included)
http://www.chalkie.org.uk		Dan's Homepages
https://ifn.mpi-cbg.de 			Biopolis Dresden Imaging Platform (BioDIP)
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )

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