My thoughts about #1 - this is still done today so days are not that old....
It is only in microscopy journals (or in microscopy lists) that you will
get called on that sort of thing.  And, as I pointed out before, it is
unimportant if the phenomenon you wish to demonstrate is consistent and
reproducible.
Rob Palmer

>1) Back in the bad old days before we had CLSMs, we took photographs of
>tissue sections that contained a z component that was limited by the
>depth of focus of the optics. We then made measurements of distances,
>lengths, sizes, etc. which in effect made xy measurements of features
>that were often separate in z. This may now be exaggerated using CLSMs
>because of the ability to get a greater z extent into the 2D projection.
>To do this on a more exact basis would require a huge number of
>calculations because it would require measuring withing many members of
>the z series of images. Although theoretically possible, it sounds very
>impractical to me.
>