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Hi Thomas,
Thank you for that idea. I will look into it and report back!

Paul

Paul Herzmark
Specialist
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Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA  94720-3200
(510) 643-9603
(510) 643-9500 fax


On Tue, Jul 17, 2012 at 12:32 AM, Horn Thomas <[log in to unmask]>wrote:

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>
> Hello Paul,
> this is just an idea, haven't tried it- make an artificial gill: use a
> blood dialysis cartridge and make two independent fluid circuits: one
> closed loop in which you insert a gas-wash bottle (so it will foam in this
> circuit), and one that flows through the fibers into your observation
> chamber.
> Just make sure that both liquids are identical and fresh.
> Best regards,
> Thomas
>
>
> Dr. Thomas Horn,
> The Single Cell Unit, U1.46
> Department of Biosystems Science and Engineering (D-BSSE)
> Swiss Federal Institute of Technology Zurich (ETH)
> Mattenstrasse 26
> CH 4048 Basel
> Switzerland
> Phone: +41 61 387 3373
> mail: [log in to unmask]
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Deanne Veronica Catmull
> Sent: Freitag, 13. Juli 2012 07:46
> To: [log in to unmask]
> Subject: Re: oxygenate medium
>
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>
> I don't know how your experimental set up works but have you tried using a
> stirrer bar and a plate to slowly stir the main media reservoir whilst
> gassing? You could probably avoid the foaming by turning down the gas flow.
> It should not affect the media as the stirring would be helping to
> distribute the gas evenly through the media. You may also want to fit
> proper air filters onto the media bottle which will help regulate the
> pressure inside the bottle which may assist.
>
> Regards,
> Deanne.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Paul Herzmark
> Sent: Tuesday, 19 June 2012 9:09 AM
> To: [log in to unmask]
> Subject: oxygenate medium
>
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> Hi all,
>
> We do 2P microscopy of thick tissue explants. To deliver appropriate
> oxygen and carbon dioxide levels to the deep parts of the tissue we bubble
> the medium with 95% O2 and 5% CO2. That keeps the cells as happy as when
> they are vascularized.
>
> Now we want to do the experiments with serum added to the medium, but the
> extra protein causes the bubbling medium to foam too much.
>
> Any suggestions on how to increase the dissolved O2 and CO2 without the
> foaming?
> Also, any ideas on how to monitor the resulting O2 levels?
>
> Thanks so much.
>
>
> Paul Herzmark
>
> [log in to unmask]
> Department of Molecular and Cell Biology
> 479 Life Science Addition
> University of California, Berkeley
> Berkeley, CA  94720-3200
> (510) 643-9603
>