Tarik,

I have custom made "flow cells" which I guess are akin to "incubation
chambers."  The flow cell creates a water tight chamber approx 300 microns
deep by 24mm x 60mm area or 41mm x 61mm.  It uses a #1 glass coverslip.  It
could be used for your application, but I use it for creating biofilms on
either the glass coverslip or a material insert.

Hope this helps.

Cheers,

-Guy

**************************************
Guy Cook
President
Bacterin Inc.
P.O. Box 6743
Bozeman, MT 59771-6743
(406) 582-8184
[log in to unmask]
http://www.bacterin.com
*************************************

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> From: tarik haydar <[log in to unmask]>
> To: [log in to unmask]
> Subject: thick specimen mounting
> Date: Monday, February 22, 1999 4:10 PM
>
> Hi Folks,
>
> I need to mount thick specimens for confocal microscopy - I use aqueous
> mounting media, so I also need to be able to seal the coverslip on the
> slide.  Does anyone know of commercial coverslips for thick tissue?  I
> need something that creates a chamber 200-300 microns high (I would
> rather not have build each one of these with spacers, etc.).  Has anyone
> ever used these "Coverwell Incubation Chambers" that RPI sells?  The
> "coverslip" they use is plastic and they couldn't even guess as to the
> refractive index or spectral absorbance.  Lately I've been reduced to
> tearing these plastic coverslips off of the gaskets they come attached
> to and replacing 'em with glass coverslips.  This takes a long time,
> though, and I would much rather do it more simply if any of you out
> there can help.
>
> Thanks in advance!
>
> --
> Tarik Haydar, Ph.D.
> Yale University Medical School
> Section of Neurobiology
> Sterling Hall of Medicine, C-319
> 333 Cedar Street
> New Haven, CT 06520
>
> Phone: (203) 785-5418
> Fax: (203) 785-5263
> [log in to unmask]