Dear Confocalists.

I wanted to know what people do to create RGB movies from their Leica_NT
3D or timelapse files.  These files are concatenated TIFF (I guess
similar to NIH Image stacks) that store the 3 channels in G1...Gn,
R1...Rn, B1...Bn order.

We want to create colour QT movies, each frame combining the 3 equvalent
RGB frames (ie G1R1B1...GnRnBn).

I've tried different options to get there.  So far the quickest is using
Imaris to export the data as series of RGB TIFF, and then use Graphic
Converter (on a Mac) to create a colour QT movie from these files.

I want to bypass the IMARIS step. Is there a program that can be told
that the serie of tiff images presented to it are actually 2 or 3
channels and that they should be combined together?

If tried presenting the original Leica_NT data to graphic converter.
It converts the TIFF series to QT, but it leaves the channels one after the
other, in sequence.

Ideally, I want to tell the program that out of the 30 images in that
file, 1-10 are Green, 11-20 are Red and 21-30 are Blue, so thay can be
combined accordingly.

Are any plug-ins for Photoshop or Graphic Converter that could do what I
want?

Thanks,

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Dr. Stamatis Pagakis                               [log in to unmask]
Confocal and Image Analysis Laboratory              Tel: +44 (0)181 913 8675
Division of Membrane Biology                Switchboard: 0181 9593666 x2621
National Institute for Medical Research         Message: x2219, x2622
The Ridgeway, Mill Hill, London NW7 1AA             FAX: +44 (0)181 906 4477