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*****

Forgot to mention:
Webcams are always RGB with an IR-cut filter (can sometimes be removed),
with all the associated advantages and disadvantages. Also, most webcams
use lossy compression (like MJPEG), so shouldn't be used for
anything quantitative...
zdenek

On Mon, Aug 21, 2023 at 2:24 PM Zdenek Svindrych <[log in to unmask]> wrote:

> Hi Marco,
>
> Cheap, but not exactly straightforward is using a webcam.
>
> First, the webcam. Nothing super fancy is needed, but the lens needs to be
> something more than just a tiny pinhole. The camera pupil should be at
> least 2 mm or so, so you can match it with the eyepiece exit pupil. These
> webcams typically have focusing capability, as the bigger aperture means
> shallower depth of focus. It's great if the webcam software allows some
> control, like exposure and focus. E.g. Logitech C615 can be found on eBay
> for less than $20.
>
> Second, more tricky, you need to align the eyepiece pupil and the webcam
> lens pupil with sub-millimeter precision. That's where you need to get
> crafty with lots of hot glue or some 3D printing. It's great if you have a
> spare eyepiece to which you can glue the webcam permanently.
>
> Somewhat better (and more expensive) solution might be a C-mount (or
> CS-mount) machine vision camera plus an eyepiece-to-Cmount adapter. Here
> you need to consider the magnification of the adapter so it matches well
> the sensor size of the camera. E.g. for a "1/3 inch" sensor (6 mm diagonal)
> you need 0.33x adapter magnification to get the best of the 18 mm FN (field
> number) of the microscope. This should be mechanically more sturdy, and you
> have better control of the camera in terms of exposure time, gain, etc.
>
> Best, zdenek
>
>
>
>
> On Fri, Aug 18, 2023 at 9:15 AM Marco Tjakra <[log in to unmask]>
> wrote:
>
>> *****
>> To join or leave the confocal microscopy listserv or to change your email
>> address, go to:
>> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear all,
>>
>> Hope you are all doing well and had a nice summer.
>> We are planning to set up a dissolution experiment in our light
>> microscope (leitz laborlux s leica).
>> The idea (and the light microscope) is really simple, we just need to
>> find an eyepiece/ocular lens that also functions as video camera (with
>> software) that can record the process.
>> Any recommendations please? Economic option would be much appreciated 😊
>> Thank you for your help!
>>
>> Sincerely,
>> Marco
>>
>>
>>
>>
>>
>>
>>
>>
>> När du har kontakt med oss på Uppsala universitet med e-post så innebär
>> det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör
>> det kan du läsa här: http://www.uu.se/om-uu/dataskydd-personuppgifter/
>>
>> E-mailing Uppsala University means that we will process your personal
>> data. For more information on how this is performed, please read here:
>> http://www.uu.se/en/about-uu/data-protection-policy
>>
>
>
> --
> --
> Zdenek Svindrych, Ph.D.
> Research Scientist - Microscopy Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
>
>

-- 
-- 
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth