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Date: | Mon, 11 Feb 2008 11:50:33 -0600 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Glen MacDonald wrote:
> Nyquist sampling at 2.3 times the object frequency is adequate for
> detection, but to resolve the point to point Rayleigh resolution, you
> need to double it again to 4.6X the object frequency, especially if
> deconvolving.
I may be wrong on this, but unless they fixed it sometime while my back
was turned, the only "true" zooms possible on a BioRad are 1, 2, 4 and
8x. Intermediate values requires double-scanning some lines. Using
such a value can result in artifacts when deconvolving.
Take care!
Martin Wessendorf
--
Martin Wessendorf, Ph.D. office: (612) 626-0145
Assoc Prof, Dept Neuroscience lab: (612) 624-2991
University of Minnesota Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009
Minneapolis, MN 55455
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