Hi Mancini
The other live cell imaging system we use for energy transfer and cell
migration using GFPs is the Inovision corporation imaging system (Isee
software) and it is Silicon Graphics based system. we went with the system
because of the speed of data acquisition, network operation for time-lapse
recording.This company based in Research Triangle park, Raleigh, NC.
The telephone number (919) 788-9998. or http://www.inovis.com
Also you should consider as Jeff suggested for the presentation of the
data, such as VCR, slide maker, etc.
periasamy
At 23:23 6/22/98 -0400, you wrote:
>I suggest go confocal if you can afford it. If you do, check out Noran. (I
>might be able to suggest others if Noran wasn't the only video rate confocal
>I've seen in the last few years.)
>
>For nonconfocal, we have used MetaFluor and MetaMorph software by Universal
>Imaging and their software works very well. You may already know they have
>another product designed for GFP applications. However, I'm not sure they
>can satisfy your requirements for speed.
>
>One practical thing that I would include in the budget request is a couple
>of nice SuperVHS player/recorders. We use JVC models and they're a good
>bang for the buck. Another is software and computer for video editing. My
>choice here would be an SGI O2 computer, because of its speed when handling
>video data. We have used Adobe Premiere on a loaded Win95 machine with a
>video I/O card and that also does the job.
>
>Hope this helps.
>
>Jeff Reece
>Biomedical Engineer
>NIEHS Confocal Microscopy Center
>National Institute of Environmental Health Sciences
>P.O. Box 12233, MD F2-02
>Research Triangle, NC 27709
>ph: (919) 541-0311
>fax: (919) 541-1898
>[log in to unmask]
>
>
>> ----------
>> From: Mancini[SMTP:[log in to unmask]]
>> Reply To: Confocal Microscopy List
>> Sent: Monday, January 01, 2001 12:43 PM
>> To: [log in to unmask]
>> Subject: live imaging options
>>
>> This has probably been bashed around previously, but I would appreciate
>> comments (to the board or directly to me) regarding people's experience
>> with various "live imaging" systems that are on the market. We are
>> planning an equipment grant to address the needs of imaging a variety of
>> cell culture models in the live state, principally aiming for
>> bioluminescent proteins (GFP, BFP, etc.).
>>
>> Our main interest is in intranuclear movement that preliminary studies
>> suggest require tens of sections (or more) per sec. Of course there will
>> be probably some loss of resolution, but the dynamics are such that force
>> the need for speed. Also, versatility with filters/lasers?/etc and
>> excellent phase or DIC is required.
>>
>> Any comments on systems to recommend/avoid would be greatly appreciated.
>>
>> Sincerely,
>>
>> Michael A. Mancini, Ph.D.
>> Assistant Professor
>> Director, Integrated Microscopy Core
>> Department of Cell Biology
>> Baylor College of Medicine
>> Houston, TX 77030
>> 713 798 8952
>> [log in to unmask]
>>
>
>
Ammasi Periasamy, Ph.D.
Director, Center for Cellular Imaging
University of Virginia
Department of Biology, Gilmer Hall (039)
Charlottesville, VA 22903
E-mail: [log in to unmask]
http://minerva.acc.virginia.edu/~biology/Fac/
Tel: (804) 243-7602; Lab.: (804) 982-4869; Fax: (804) 982-5210
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