*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

On 8/20/12 4:45 AM, George McNamara wrote:
>
> If this is correct, then you are using the scanning mirrors to create
> the image. Since you are not using a point illumination source (the
> focused lasers), you will probably be better off opening the pinhole to
> collect more light. However, on the spectral confocal systems I am
> familiar with (Zeiss LSM710 and Leica SP5 and SP1) opening the pinhole
> compromises the spectral resolution.
>

In the Nikon C1 and A1 spectral, the spectral unit is on the other side 
of a fiber, while the pinhole is in the scanhead. Therefore, spectral 
resolution should be independent of pinhole size.

--aryeh
-- 
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-7384051