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DPX has worked for us for Cy2, Cy3 (and any other red fluorophore that
we've tried), Bodipy--anything except FITC.  Haven't tried any of the
Alexa dyes.

Martin Wessendorf

Xuejun Sun wrote:

> I believe DPX will do. I used it with Cy3 which survived dehydration. But
> you might need to test other fluorochromes if you want to use it.
>
> Xuejun
>
> -----Original Message-----
> Dear list members,
>
> which are the embedding media with highest refractive index (r.i. 1.48 to
> 1.52) but still compatible with standard fluorochromes / fluorescent
> proteins?
>
> Ideally, the refractive index would match the r.i .of immersion oil (e.g.
> ZEISS Immersol 518 F: r.i. 1.518).
>
> Any reference welcome.
>
> Guenter
>
> ------------------------------------------
> Dr. Guenter Giese
> Light Microscopy Facility
> Dept. of Biomedical Optics, MPI fuer Medizinische Forschung
> Jahnstr. 29, D-69120 Heidelberg, Germany
> Phone (+49) 6221-486-360 (Fax: -325)
> e-mail: [log in to unmask]
> http://www.mpimf-heidelberg.mpg.de/~ggiese/lightmicro

--
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