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I certainly wasn't suggesting using the slide before the glue has set!
I always use a very small drop so that it doesn't reach the edge of the
coverslip since it simply won't set where it oozes out (it sets when
oxygen cannot reach it).

Of course you could also use any of the hard-setting mounting media
but they are much slower.

                                                        Guy

Larson Jeffrey wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> A caution against using "Super Glue" or any other cyanomethacrylate
> containing product anywhere near optical surfaces!  Cyanomethacrylate
> outgases as it cures or dries.  While commercially available coverslip
> mounting media like Permount consist of a resin dissolved in a solvent,
> cyanomethacrylate is a liquid.  What you smell when "Super Glue" cures
> is the same cyanomethacrylate that forms the bond.  This outgassed
> cyanomethacrylate coats and damages nearby optical surfaces.  It is
> difficult to remove cyanomethacrylate residue without damaging coatings.
>
> Jeffrey Larson
> Product Manager, Confocal Systems
> Nikon Instruments Inc.
> 1300 Walt Whitman Road
> Melville, NY 11747
> (631) 547-8540
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Guy Cox
> Sent: Tuesday, August 16, 2005 5:32 PM
> To: [log in to unmask]
> Subject: Re: Reflected light
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> George Macnamara wrote:
>
>>p.s. if you do not have a reflection slide, you can try to get the
>
> Leica
>
>>service engineer to come over with their's, or buy one for a few
>
> dollars
>
>>at
>>http://www.nanofilm.com <http://www.nanofilm.com>
>
>
> Or you can get one for virtually nothing by getting your
> local SEM facility to sputter coat a #1.5 coverslip.
> Superglue it to a slide (coating down) and you have
> a permanent and invaluable test specimen.
>
>                                         Guy
>
> --
> Associate Professor Guy Cox
> Electron Microscope Unit,
> University of Sydney,
> NSW 2006, Australia
>
> Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
> http://www.guycox.net

--
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net