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March 2006

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Subject:
Re: flow cytometry vs immunofluorescence microscopy
From:
Martin Spitaler <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 10 Mar 2006 06:55:54 -0500
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One major difference between FACS and microscopy is bleaching: Excitation
time on the FACS is so short, bleaching is never a problem, so if simple
dyes like PE do the job, there is no need for more expensive, but more
stable ones.

Martin Spitaler, PhD

Microscopy Facility
Imperial College / South Kensington
London SW7 2AZ
UK

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