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Dear All,
I have come across a paper that claims to show regional pH measurements
(confocal fluorescence, ratiometric, of a pH indicator) with sub-micron
resolution and no apparent noise from single images of live cells. The
images show pH gradients of about 1 pH unit over distances less than 1 um
within the cytosol. The paper is Ro & Carson 2004 JBC 279(35):37115-23. The
pH gradients are shown on the cover of the 27th August edition of JBC.
The PubMed link is:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?
cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15192115
If you look at the inset to Fig 1C it shows a region 5um long with pH 7.5
regions sitting right next to pH 6.5 regions.
Are these sorts of images, with very high resolution and no noise possible?
Does anyone feel able to comment?
Greetings,
Christof