We once ran Cargill's Type FF oil ("For fluorescence critical" application)
and Zeiss's immersion oil in a spectrofluorometer. If I recall correctly we
were exciting ~568 nm and looking at everything to the red. Cargill's oil
was at least 10x more fluorescent than Zeiss's. We had done this in an
effort to trace some background fluorescence using a regular microscope.
That was not the problem but we definitely did not switch to Cargill's oil.
The bottle was new but the problem could vary from lot to lot -- we never
checked.
Jim
 
>>Hello all,
>>I guess this topic has probably been discussed previously on this
>>listserver so I apologise if I'm digging up old ground.
>>Does anyone know of an appropriate brand or supplier of immersion oil for
>>confocal microscopes?
>>We have an inverted Nikon Diaphot with our Biorad MRC600.
>>I wasn't involved in the purchase of the the last lot, so I'm not too sure
>>where or who we got it from!
>>Any help or preferences would be appreciated.
>>
>>ta
>>rich.
>>
>>-----------------------------------------
>>Richard Lander
>>South Campus Electron Microscope Unit
>>c/- Pathology Department
>>Otago Medical School
>>P.O. Box 913
>>Dunedin
>>N.Z.
>>
>>Tel. National 03 479 7301  International 64 3 479 7301
>>Fax. National 03 479 7254  International 64 3 479 7254
>
>Cargille is a (the?) major supplier of immersion oils.  They have a range
>of oils with characteristics such as high viscosity (nice for an inverted
>scope) and low or fluorescence free.  Their numbers are 201-239-6633
>(voice) and 201-239=6096.
>
>
>Thomas E. Phillips, Ph.D.
>Associate Professor of Biological Sciences
>Director, Molecular Cytology Core Facility
>3 Tucker Hall
>University of Missouri
>Columbia, MO 65211
>(314)-882-4712 (voice)
>(314)-882-0123 (fax)
_________________________________________________________
James P. Slattery                  Voice: 607-254-4862
Cornell CAT in Biotechnology       FAX: 607-255-2428
Director Flow Cytometry and Imaging Facility
Director Computing Facility      E-mail: [log in to unmask]