I did consider using a far-red fluor, but my understanding is that by and
large, far-red fluors aren't as "bright" as those in the yellow/green region.



At 03:19 PM 10/10/01 +0200, you wrote:
>Anthony Mc Mahon wrote:
>>
>> Thanks for your suggestion, unfortunately I will be using
>> tetramethylrhodamine-labelled vectors in the same experiment!
>>
>
>You might consider a Cy(5) derivative -- that is, a diindocyanine dye.
>The
>fluorophore should be independent of pH since the amines are both
>tertiary,
>
>These dyes come with a variety of "tails" which are usually hydrophobic,
>since
>they are used as membrane probes. However, you might find one (or have
>one made)
>which can be conjugated to your target.
>
>--aryeh
>--
>Aryeh Weiss                          | email: [log in to unmask]
>Department of Electronics            | URL:
>http://optics.jct.ac.il/~aryeh
>Jerusalem College of Technology      | phone: 972-2-6751146
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>
>
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