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Hi Gary,

Thanks for your reply. I can make a PDMS cuvette. Did you use cubic in the
PDMS is it safe and how did it work with the refractive indices? Did you
use a dipping objective or air? And wouldn't the wobbly brain move around
when you would turn to image from different angles? I would love to learn
about this more? Thanks again.

Best regards,
Nadia

On Tue, Jan 21, 2020 at 11:38 AM Gary Laevsky <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We’ve been making cuvettes out of PDMS and PMMA, depending on the reagent.
> Put the sample in the cuvette and fill with matching reagent.
>
> Then we hang in our Bruker MuVi. Working well.
>
>
>
> Best,
>
> Gary
>
>
>
> > On Jan 21, 2020, at 3:26 AM, Nikos Ekizoglou - PlaneLight <
> [log in to unmask]> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear Nadia,
> >
> > Our QLS-scope can measure samples from < 1mm to 250mm in XYZ. We just
> put the sample in matching refractive index glass tube and that's all. No
> hanging, no gluing, no cutting and you can return the sample to your
> colleague after measurement. You are welcomed to our demo lab to Madrid
> (Spain) to measure your sample, free of charge. If you are interested,
> please send me a personal email.
> >
> > Best regards
> >
> > --
> > *Nikos Ekizoglou*
> >
> > *Plane Light S.L.*
> > Edificio CTM, O-309
> > 28053, Madrid
> > Tel. +34 675 966 041
> > /www.planelight.net/
> >
> >> El 21/01/2020 a las 8:33, Nadia Halidi escribió:
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> Post images on http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> Dear Lightsheet people,
> >>
> >> Someone brought me mouse brain samples cleared in cubic and wants to
> image
> >> the entire brain. They have scanned the same brain with CT first then
> went
> >> ahead and cleared it with cubic. They want to image the entire Brain
> >> without out any loss to compare CT vs lightsheet data. They are
> desperate
> >> to image.
> >>
> >> I have a Z1 lightsheet, I would usually need to "hang" the samples.
> Their
> >> condition is that they want to image the entire brain. The sample is too
> >> wobbly. I have run out of ideas and about to refer them somewhere where
> >> they place the sample down and rely on gravity, no hanging (like
> LaVision).
> >> But before I do, I thought to ask here if any of you have any
> suggestions,
> >> or perhaps have already succeeded. Thank you.
> >>
> >> Best regards,
> >> Nadia Halidi
> > --
> > *Nikos Ekizoglou*
> >
> > *Plane Light S.L.*
> > Edificio CTM, O-309
> > 28053, Madrid
> > Tel. +34 675 966 041
> > /www.planelight.net
> > /
>