Hi Waldo,
 
Your other choice is to get a Sykes-Moore chanmber made by Belco. The are a simple chamber with a rubber seal and a screw on top that holds it all together. We use them daily and they work great. You can grow your cells on the coverslips then just mount them in the chamber and image happily. The only problem i have had with them is that you cannot usually image to whole coverslip. When mounted the cover slip is recessed a little bit which means large objectives can only focus on the middle area of the cover slip.
 
A big thanks to Steve Cody for buying these in for the Centre. Also I have no comercial intrsts with Belco.
 
 
Cheers
 
Cam
 
 
 
 
Cameron J. Nowell
Microscpy Manager
Central Resource for Advanced Microscopy
Ludwig Insttue for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
 
Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104
 
http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
 

________________________________

From: Confocal Microscopy List on behalf of Waldo Schmidt
Sent: Wed 26/08/2009 11:31 PM
To: [log in to unmask]
Subject: Coverslip on coverslip - can cells inbetween be imaged?



We have the problem of our cells differentiating well on specific coverslips,
and not those of mattek dishes. In the meantime of trying to identify other
dishes, we continue to use coverslips.

We image just with a dipping lens, but would like some more resolution.

Is it possible to mount the "coverslip with cells" upsidedown onto a new
mattek dish and then image it using an inverted microscope with a water
immersion lens?

Is there another way around this?

Regards