You can also use Congo red to stain walls.  You can by coriphosphene to Pfaltz
& Bauer wich webpage is attached to this mail.

Liz Nickless wrote:

> >
> >Hello,
> >
> >We are trying to get a 3-dimensional picture of a plant meristem using our
> Confocal Microscope. The plant that we are studying is very hairy and seems
> to be very resinous or waxy. We are having trouble getting fixatives into
> the tissue and also clearing the tissue. The stains we have tried are
> Saffranin and Fast green. These both fluoresce but neither is getting into
> the tissue very well. I have read that Coryphosphine O is a good plant cell
> wall stain that fluoresces, but I have not had any success in tracking down
> a supplier. If anyone can help me with a supplier for this or has done
> confocal work on this sought of sample I would very much appreciate some
> advice. I have done work on maize meristems that work quite well with
> propidium iodide. This only stains DNA/RNA etc so is not suitable for our
> purpose with the other tissue as we need to see cell walls.
> >Please, can anyone Help?
> >
> >Thankyou
> >
> >Liz
> >



--

MSc Franco Pedreschi
PhD Student
Pontificia Universidad Católica de Chile
Department of Chemical Engineering and Bioprocesses
Vicuña Mackena 4860.  PO BOX 306
e-mail:[log in to unmask]
Phone:(56-2)6864264
Fax:(56-2)6865803