Jizhong Wang writes:
 
> Now I am looking for a new fluorescence dye for intracellular injection
> in fixed brain slice, after injection I want to make DAB immunoreaction.
> With LY (luciffer yellow) I could get very nice filled neuron, but the
> immunoreaction was always unsuccessful. Somebody told me that the LY
> blocked the immunoreaction.
 
There have been many papers in which Lucifer-stained neurons have been stained
immunocytochemically (althought the staining was generally done using
fluorescence methods).  What also has been reported, though, is that DAB
quenches fluorescence.  Thus your friend may have had the story backwards--it's
probably not that Lucifer will interfer with DAB.  Rather, it's that DAB will
probably interfer with Lucifer.
 
Good Luck!
 
Martin Wessendorf PhD, Asst Professor                [log in to unmask]
Dept Cell Biology and Neuroanatomy                      (612) 624 2991 (voice)
Confocal Microscopy Facility                              (612) 624 8118 (FAX)
University of Minnesota
Minneapolis, MN  55455