Jay,

Changing the image resolution from 72dpi (screen) to 300dpi (print) ought to
be about as easy as converting from inches to centimeters.  The Photoshop
image size dialog box is powerful and a bit of landmine.  Which is why I
wrote this handout for our local users:
http://swehsc.pharmacy.arizona.edu/exppath/resources/pdf/Photoshop_Image_Siz
e_dialog_box.pdf  "Potentially the most dangerous dialog box in Adobe
PhotoshopT"

672x512 pixels is approximately 2.25in by 1.7in at 300dpi.

For the purposes of printing bitmapped images 300dpi is pretty much the same
as 300ppi.

I'm not much of an Adobe Illustrator guy, so I'll leave it to others to
comment on.

At the risk of seeming self-promoting, I'd like to pass on this article that
I wrote, which just became available online last week:

Avoiding Twisted Pixels: Ethical Guidelines for the Appropriate Use and
Manipulation of Scientific Digital Images
http://www.springerlink.com/content/00311qw26613m261/?p=499e7ec2a1174fad863e
7b597298edd4&pi=0

Best regards,
Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Assistant Scientific Investigator
Dept. of Cell Biology & Anatomy, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  AHSC 4212         email: [log in to unmask]
voice:  520-626-2824       fax:  520-626-2097

http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Jay Vyas
Sent: Monday, June 28, 2010 1:23 PM
To: [log in to unmask]
Subject: Preparing figures for publication --PPI vs DPI

Hi all--

We have a spinning disck confocal microscope that uses Metamorph. We are 
in the process of preparing figures for a manuscript. We consulted with the 
information for authors for the desired journal and was confused to learn
that 
the images must be 300 DPI (dots per inch). I understand that images are 
measured using PPI (pixels per inch) and DPI is not the same as PPI. When I 
consulted with the copy editor of the journal, I did not get useful
information. 
I will assume that they wish to have images at 300 PPI

How do you take images from Metamorph (raw data) and end up with a TIFF 
ready for publication?

I propose to do the following
1. Open raw data image in photoshop. It is 672 pixels by 512 pixels.
2. adjust contrast
3. If I open the image information, it reads 72 pixels/inch
4. Crop image to select cell
5. SAve image as tiff file
6. Open adobe illustrator
7. place file in adobe illustrator
8. scale image to final size (this typically reduces the physical dimensions
of 
the image by 50%)
9. annotate file with arrows and labels as necessary
10. Set raster effect to 300ppi on the print dialog.

I would love input from the group to see if I have done anything here that 
would be considered suboptimal (or wrong...).