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Hi, again

Yes, as a microscopy teacher for over 25 years, I strongly agree.  I 
have personally tested this system with a resolution target below 
90nm.  Similar tests are on the Cytoviva website.

-B

At 11:55 PM 9/6/2006, Mark Cannell wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>I think that one needs to be careful about the term resolution. 
>There is a huge
>difference between detection and resolution. After all, it is easy to detect
>distant stars but (generally) none of them are actually resolved.
>
>Regards Mark
>
>Quoting Barbara Foster <[log in to unmask]>:
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Hi, Lars
> >
> > Before trying all the fancy stuff like TIRF, etc, there are two other
> > alternatives that I would investigate:
> > a. CytoViva (which pushes the resolution of light microscopy well
> > below 100nm - HONESTLY!), now comes equipped with a version to work
> > with fluorescence.  The results have been impressive (see
> > www.cytoviva.com).  CytoViva also has the ability to optically
> > section, which I think you might find helpful in this
> > application.  (By the way, CytoViva just won an R&D 100 award this year)
> > b. I also had a chance to work some time back with a client in Europe
> > who has invented an intriguing slide apparatus that creates a
> > localized plasmon field  that gives results similar to TIRF with less
> > hassle (www.lumiscence.com).
> >
> > Hope this is helpful,
> >
> > Barbara Foster
> >
> > Microscopy/Microscopy Education
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> > semester? Call us today to learn more about "Optimizing LIght
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> > At 11:46 AM 9/7/2006, Engstrom, Lars wrote:
> > >Search the CONFOCAL archive at
> > >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> > >Hello all -
> > >Does anyone have experience imaging DiI-LDL in live cells with
> > >either wide field or confocal?
> > >We have had some success w/ incubating at 4C prior to imaging.
> > >We are mostly interested at what's happening at the membrane. When
> > >imaged w/ FM 4-64 we see some co-localization with the DiI-LDL but
> > >the far majority is not.
> > >We are having difficulty resolving the DiI-LDL at early time points.
> > >I've found some clathrin-mediated endocytosis papers using labeled
> > >LDL, a GFP-fusion to clathrin and confocal. I also found a couple
> > >papers using TIRF, is that a better platform to be using?
> > >Another lab is working on making some fusion vectors/proteins but I
> > >wanted to get a start on the imaging while waiting for them.
> > >Any suggestions on approach would be appreciated.
> > >Thanks for your time.
> > >-Lars
> > >
> > >
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